P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09093

Novus Biologicals, part of Bio-Techne
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NBP3-09093
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Applications

Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AK4

Format

Low Endotoxin, Azide and BSA Free

Concentration

1 mg/ml

View all P-Selectin/CD62P Antibodies »

Product Summary for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free

Immunogen

Human platelets

Specificity

The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093]

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093]

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093] - Separation of human thrombocytes (red-filled) from CD62P negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62P (AK4) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.
Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093]

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093]

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093] - Surface staining pattern of human peripheral blood stained using anti-human CD62P (AK4) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.

Applications for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1.5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Formulation

PBS, 0.2 um filter sterilized

Format

Low Endotoxin, Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P-Selectin/CD62P

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Gene Symbol

SELP

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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