NB100-65391 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for P-Selectin/CD62P Antibody (Psel.KO.2.12) [DyLight 550]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: P-Selectin/CD62P

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Gene Symbol

SELP

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (Psel.KO.2.12) [DyLight 550]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for P-Selectin/CD62P Antibody (Psel.KO.2.12) [DyLight 550]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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Flow Cytometry

P-Selectin/CD62P Antibody (Psel.KO.2.12) [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65391R

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

View all P-Selectin/CD62P Antibodies »

Product Summary for P-Selectin/CD62P Antibody (Psel.KO.2.12) [DyLight 550]

Immunogen

Reactivity Notes

Localization

Specificity

Clonality

Host

Isotype

Applications for P-Selectin/CD62P Antibody (Psel.KO.2.12) [DyLight 550]

Flow Cytometry

Immunohistochemistry

Immunohistochemistry-Frozen

Immunoprecipitation

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: P-Selectin/CD62P

Alternate Names

Gene Symbol

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (Psel.KO.2.12) [DyLight 550]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for P-Selectin/CD62P Antibody (Psel.KO.2.12) [DyLight 550]