P-Selectin/CD62P Antibody (Psel.KO.2.12)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65391

Novus Biologicals, part of Bio-Techne
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NB100-65391
NB100-65391-0.025mg
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Conjugate
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Alexa Fluor 350
NB100-65391AF350
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Alexa Fluor 405
NB100-65391AF405
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Alexa Fluor 488
NB100-65391AF488
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Alexa Fluor 532
NB100-65391AF532
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Alexa Fluor 594
NB100-65391AF594
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Alexa Fluor 647
NB100-65391AF647
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Alexa Fluor 700
NB100-65391AF700
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Alexa Fluor 750
NB100-65391AF750
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Allophycocyanin
NB100-65391APC
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Allophycocyanin/Cy7
NB100-65391APCCY7
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Biotin
NB100-65391B
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CoraFluor 1
NB100-65391CL1
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DyLight 350
NB100-65391UV
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DyLight 405
NB100-65391V
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DyLight 488
NB100-65391G
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DyLight 550
NB100-65391R
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DyLight 594
NB100-65391DL594
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DyLight 650
NB100-65391C
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DyLight 680
NB100-65391FR
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DyLight 755
NB100-65391IR
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FITC
NB100-65391F
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HRP
NB100-65391H
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Janelia Fluor 525
NB100-65391JF525
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Janelia Fluor 549
NB100-65391JF549
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Janelia Fluor 585
NB100-65391JF585
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Janelia Fluor 635
NB100-65391JF635
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Janelia Fluor 646
NB100-65391JF646
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Janelia Fluor 669
NB100-65391JF669
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mFluor Violet 450 SE
NB100-65391MFV450
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mFluor Violet 500 SE
NB100-65391MFV500
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mFluor Violet 610 SE
NB100-65391MFV610
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PE
NB100-65391PE
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PE/Atto594
NB100-65391PEATT594
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PE/Cy5.5
NB100-65391PECY55
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PE/Cy7
NB100-65391PECY7
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PerCP
NB100-65391PCP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Psel.KO.2.12

Concentration

1.0 mg/ml

View all P-Selectin/CD62P Antibodies »

Product Summary for P-Selectin/CD62P Antibody (Psel.KO.2.12)

Immunogen

CD62P transfected 300.19 cells

Reactivity Notes

Reacts with Human. Cross reacts with Goat, Rat and Sheep.

Localization

Membrane; single pass type I membrane protein.

Specificity

NB100-65391 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for P-Selectin/CD62P Antibody (Psel.KO.2.12)

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free [NB100-65391]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free [NB100-65391]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.12) [NB100-65391] - Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control. Figure B . FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD62P. All experiments performed on human blood gated on single platelets activated with thrombin, in the presence of 10% Human serum.

Applications for P-Selectin/CD62P Antibody (Psel.KO.2.12)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P-Selectin/CD62P

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Entrez Gene IDs

6403 (Human)

Gene Symbol

SELP

UniProt

P16109

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (Psel.KO.2.12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for P-Selectin/CD62P Antibody (Psel.KO.2.12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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