p107 Antibody (1A5)

Novus Biologicals, part of Bio-Techne | Catalog # H00005933-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p107 Antibodies »

Product Specifications

Immunogen

RBL1 (AAH32247, 905 a.a. ~ 1014 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IDCDLEDATKTPDCSSGPVKEERGDLIKFYNTIYVGRVKSFALKYDLANQDHMMDAPPLSPFPHIKQQPGSPRRISQQHSIYISPHKNGSGLTPRSALLYKFNGSPSKVR

Specificity

RBL1 - retinoblastoma-like 1 (p107)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for p107 Antibody (1A5)

Western Blot: p107 Antibody (1A5) [H00005933-M01]

Western Blot: p107 Antibody (1A5) [H00005933-M01]

Western Blot: p107 Antibody (1A5) [H00005933-M01] - Analysis of RBL1 expression in transfected 293T cell line by RBL1 monoclonal antibody (M01), clone 1A5.Lane 1: RBL1 transfected lysate (Predicted MW: 111.54 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: p107 Antibody (1A5) [H00005933-M01]

Proximity Ligation Assay: p107 Antibody (1A5) [H00005933-M01]

Proximity Ligation Assay: p107 Antibody (1A5) [H00005933-M01] - Analysis of protein-protein interactions between MYBL2 and RBL1. Mahlavu cells were stained with anti-MYBL2 rabbit purified polyclonal 1:1200 and anti-RBL1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for p107 Antibody (1A5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p107

The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

cellular protein 107,107 kDa retinoblastoma-associated protein, CP107, p107MGC40006, PRB1, retinoblastoma-like 1 (p107), retinoblastoma-like protein 1

Entrez Gene IDs

5933 (Human)

Gene Symbol

RBL1

OMIM

116957 (Human)

UniProt

AAH32247 (Human)

Additional p107 Products

Product Documents for p107 Antibody (1A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p107 Antibody (1A5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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