Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CTNND1/4501

Format

Azide and BSA Free

Concentration

1 mg/ml

View all p120-catenin Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa138-276) of human p120-catenin (exact sequence is proprietary) (Uniprot: O60716)

Localization

Cell Surface, Cytoplasm

Specificity

Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120 catenin binds E-cadherin at a juxta-membrane site. The complex stabilizes tight junctions. In the cell, p120 catenin localized to the E-cadherin/catenins cell adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins. p120 is a proliferation-associated nucleolar protein found in most human malignant tumors, but not in resting normal cells. In colorectal cancer the altered localization of p120 catenin corresponds with loss of cytoplasmic localization of E-cadherin. Studies have shown accurate categorization of ductal vs. lobular neoplasia in the breast was achieved with p120 staining. p120 expression further clarifies the separation of low-grade ductal carcinoma in situ from lobular neoplasia. Studies also have shown that altered expression of p120 catenin antibody predicts poor outcome in invasive breast cancer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07801).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free

Immunohistochemistry-Paraffin: p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free [NBP3-08614]

Immunohistochemistry-Paraffin: p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free [NBP3-08614]

Immunohistochemistry-Paraffin: p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free [NBP3-08614] - IHC analysis of formalin-fixed, paraffin-embedded human colon. p120-catenin antibody (CTNND1/4501) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free [NBP3-08614]

Immunohistochemistry-Paraffin: p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free [NBP3-08614]

Immunohistochemistry-Paraffin: p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free [NBP3-08614] - Negative tissue control. IHC analysis of formalin-fixed, paraffin-embedded human brain stained with p120-catenin antibody (CTNND1/4501) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.

Applications for p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: p120-catenin

The alpha, beta, delta, and gamma -catenins are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. alpha-catenin, a 102 kDa protein, interacts with E-cadherin associated protein, and also associates with other members of the cadherin family, such as N-cadherin and P-cadherin. The 92 kDa beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also complexes with the tumor suppressor protein APC. delta-catenin interacts with Presenilin 1 and is expressed in the brain. The gene encoding delta-catenin maps to human chromosome 5p15.2. A hemizygous loss of the gene encoding delta-catenin leads to the mental retardation associated with Cri-du-Chat syndrome. gamma-catenin, also known as plakoglobin, is an 80-88 kDa protein that binds alpha-catenin and N-cadherin. In addition, the transmembrane phosphatase PTPm associates with catenin/cadherin complexes and may regulate complex signaling.

Alternate Names

Cadherin-associated Src substrate, CAS, catenin (cadherin-associated protein), delta 1, catenin delta-1, CTNND, KIAA0384P120CAS, p120, p120 catenin, p120(CAS), p120(CTN), p120cas, p120ctn

Gene Symbol

CTNND1

Additional p120-catenin Products

Product Documents for p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p120-catenin Antibody (CTNND1/4501) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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