p19ARF/CDKN2A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB200-106

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all p19ARF/CDKN2A Antibodies »

Product Specifications

Immunogen

Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF/CDKN2A. [Swiss-Prot #Q64364]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31827530).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p19ARF/CDKN2A Antibody - BSA Free

Western Blot: p19ARF/CDKN2A AntibodyBSA Free [NB200-106]

Western Blot: p19ARF/CDKN2A AntibodyBSA Free [NB200-106]

Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/ Immunofluorescence: p19ARF/CDKN2A Antibody - BSA Free [NB200-106]

Immunocytochemistry/ Immunofluorescence: p19ARF/CDKN2A Antibody - BSA Free [NB200-106]

Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: p19ARF/CDKN2A Antibody - BSA Free [NB200-106]

Immunohistochemistry: p19ARF/CDKN2A Antibody - BSA Free [NB200-106]

Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
View 1 more images

Applications for p19ARF/CDKN2A Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Frozen

1 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

2 ug/ml
Application Notes
In WB a band can be seen at approximately 19 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 3 using NB200-106 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p19ARF/CDKN2A

p19ARF (mouse) and p14ARF (human) has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.

Alternate Names

alternative reading frame, ARF, ARF-INK4a, CDK4I, CDKN2A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4), cyclin-dependent kinase inhibitor 2A, isoform 3, cyclin-dependent kinase inhibitor 2A, isoforms 1/2, cyclin-dependent kinase inhibitor protein, INK4, INK4a, Ink4a/Arf, INK4a-ARF, mitochondrial smARF', MTS1, p14, p14ARF, P16, p16(INK4a), p16-INK4, P16INK4A, p16-INK4a, P19, p19, p19Arf, Pctr1

Gene Symbol

Cdkn2a

Additional p19ARF/CDKN2A Products

Product Documents for p19ARF/CDKN2A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p19ARF/CDKN2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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