p21/CIP1/CDKN1A Antibody (SPM306)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44526
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526]](https://resources.bio-techne.com/images/products/p21-CIP1-CDKN1A-Antibody-SPM306-Immunohistochemistry-Paraffin-NBP2-44526-img0001.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse (Negative), Rat (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # SPM306
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human recombinant p21/CIP1/CDKN1A protein (Uniprot: P38936)
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Nuclear
Specificity
This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47782)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for p21/CIP1/CDKN1A Antibody (SPM306)
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526]
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526] - Human Colon Carcinoma stained with p21 Monoclonal Antibody (SPM306).
Western Blot: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526] -
Western Blot: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (SPM306).Applications for p21/CIP1/CDKN1A Antibody (SPM306)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
2-4 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: p21/CIP1/CDKN1A
Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Alternate Names
CDKN1A, CIP1
Gene Symbol
CDKN1A
UniProt
Additional p21/CIP1/CDKN1A Products
Product Documents for p21/CIP1/CDKN1A Antibody (SPM306)
Product Specific Notices for p21/CIP1/CDKN1A Antibody (SPM306)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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