p21/CIP1/CDKN1A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03297

Novus Biologicals, part of Bio-Techne
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NBP3-03297-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p21/CIP1/CDKN1A Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human p21/CIP1/CDKN1A (NP_000380.1). MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p21/CIP1/CDKN1A Antibody - BSA Free

p21/CIP1/CDKN1A Antibody - BSA Free

Western Blot: p21/CIP1/CDKN1A Antibody - BSA Free [p21/CIP1/CDKN1A] -

Western Blot: p21/CIP1/CDKN1A Antibody - BSA Free [p21/CIP1/CDKN1A] - Western blot analysis of various lysates using p21/CIP1/CDKN1A Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
p21/CIP1/CDKN1A Antibody - BSA Free

Immunoprecipitation: p21/CIP1/CDKN1A Antibody - BSA Free [p21/CIP1/CDKN1A] -

Immunoprecipitation: p21/CIP1/CDKN1A Antibody - BSA Free [p21/CIP1/CDKN1A] - Immunoprecipitation of p21/CIP1/CDKN1A from 200 ug extracts of MCF7 cells was performed using 0.5 ug of p21/CIP1/CDKN1A Rabbit pAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using p21/CIP1/CDKN1A Rabbit pAb at a dilution of 1:500.
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody - BSA Free [NBP3-03297]

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody - BSA Free [NBP3-03297]

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody [NBP3-03297] - Human breast cancer using [KO Validated] CDKN1A/p21CIP1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for p21/CIP1/CDKN1A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p21/CIP1/CDKN1A

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p21/CIP1/CDKN1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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