Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken, Guinea Pig, Rabbit, Yeast

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # JJ6

Concentration

1 mg/ml

View all p23/PTGES3 Antibodies »

Product Summary for p23/PTGES3 Antibody (JJ6)

Immunogen

Recombinant human full length p23 protein

Localization

Cytoplasm

Specificity

Detects approx 23kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for p23/PTGES3 Antibody (JJ6)

Western Blot: p23/PTGES3 Antibody (JJ6) [NB110-96879]

Western Blot: p23/PTGES3 Antibody (JJ6) [NB110-96879]

Western Blot: p23/PTGES3 Antibody (JJ6) [NB110-96879] - Western Blot analysis of Human Cell lysates showing detection of p23/PTGES3protein using Mouse Anti-p23/PTGES3Monoclonal Antibody, Clone JJ6 (NB110-96879). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p23/PTGES3Monoclonal Antibody (NB110-96879) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ6) [NB110-96879]

Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ6) [NB110-96879]

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ6) [NB110-96879] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Uniform epidermal staining -some evidence of cell-cell borders. Image courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.
Immunohistochemistry: p23/PTGES3 Antibody (JJ6) [NB110-96879]

Immunohistochemistry: p23/PTGES3 Antibody (JJ6) [NB110-96879]

Immunohistochemistry: p23/PTGES3 Antibody (JJ6) [NB110-96879] - Immunohistochemistry analysis using Mouse Anti-p23/PTGES3Monoclonal Antibody, Clone JJ6 (NB110-96879). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-p23/PTGES3Monoclonal Antibody (NB110-96879) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermal, dermal, HF, muscle staining. Bright dermal staining.
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Applications for p23/PTGES3 Antibody (JJ6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Application Notes
0.5 ug/ml of p23 Antibody was sufficient for detection of p23 in 20 ug of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p23/PTGES3

p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the hsp90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the Hsp90-progesterone receptor complex along with hsp70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate

Long Name

Prostaglandin E Synthase 3 (Cytosolic)

Alternate Names

CPGES, PTGES3, TEBP

Entrez Gene IDs

10728 (Human); 56351 (Mouse)

Gene Symbol

PTGES3

UniProt

Q15185, NP_006592.3 (Human)

Additional p23/PTGES3 Products

Product Documents for p23/PTGES3 Antibody (JJ6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p23/PTGES3 Antibody (JJ6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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