Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 225501

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p27/Kip1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p27
Met1-Thr198
Accession # P46527

Specificity

Detects human, mouse, and rat p27 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for p27/Kip1 Antibody (225501) [mFluor Violet 500 SE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (225501) [mFluor Violet 500 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for p27/Kip1 Antibody (225501) [mFluor Violet 500 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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