p27/Kip1 Antibody (DCS-72.F6 + KIP1/769)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44497
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [NBP2-44497]](https://resources.bio-techne.com/images/products/p27-Kip1-Antibody-DCS-72-F6-+-KIP1-769-Immunohistochemistry-Paraffin-NBP2-44497-img0001.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [NBP2-44497]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DCS-72.F6 + KIP1/769
Concentration
0.2 mg/ml
Product Summary for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769)
Immunogen
Mouse recombinant p27/Kip1 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769) (Uniprot: P46527)
Epitope
aa 83-204
Localization
Nuclear
Specificity
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47771)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769)
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [NBP2-44497]
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [NBP2-44497] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)Applications for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: p27/Kip1
Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Alternate Names
CDKN1B, Kip1
Gene Symbol
CDKN1B
UniProt
Additional p27/Kip1 Products
Product Documents for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769)
Product Specific Notices for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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