p27/Kip1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2816

Novus Biologicals, part of Bio-Techne
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NB100-2816
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Feline (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all p27/Kip1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KRPATDDSSTQNK corresponding to internal region according to NP_004055.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for p27/Kip1 Antibody

Western Blot: p27/Kip1 Antibody [NB100-2816]

Western Blot: p27/Kip1 Antibody [NB100-2816]

Western Blot: p27/Kip1 Antibody [NB100-2816] - Human Prostate lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for p27/Kip1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 26 kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1 kDa according to NP_004055.1). ).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p27/Kip1

Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Entrez Gene IDs

1027 (Human)

Gene Symbol

CDKN1B

UniProt

NP_004055.1 (Human)

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p27/Kip1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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