P2X5/P2RX5 Antibody (1C5)
Novus Biologicals, part of Bio-Techne | Catalog # H00005026-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1C5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
P2RX5 (NP_002552, 126 a.a. ~ 224 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWCPLETSSRPEEPFLKEAEDFTIFIKNHIRFPKFNFSKSNVMDVKDRSFLKSCHFGP
Localization
Integral membrane protein.
Specificity
P2RX5 - purinergic receptor P2X, ligand-gated ion channel, 5
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for P2X5/P2RX5 Antibody (1C5)
![Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]](https://resources.bio-techne.com/images/products/P2X5-P2RX5-Antibody-1C5-Western-Blot-H00005026-M01-img0006.jpg "Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]")
Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]
Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Analysis of P2RX5 expression in transfected 293T cell line by P2RX5 monoclonal antibody (M01), clone 1C5.Lane 1: P2RX5 transfected lysate(46.42 KDa).Lane 2: Non-transfected lysate.![Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]](https://resources.bio-techne.com/images/products/P2X5-P2RX5-Antibody-1C5-Western-Blot-H00005026-M01-img0004.jpg "Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]")
Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]
Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Analysis of P2RX5 over-expressed 293 cell line, cotransfected with P2RX5 Validated Chimera RNAi ( Cat # H00005026-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with P2RX5 monoclonal antibody (M01), clone 1C5 (Cat # H00005026-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Detection limit for recombinant GST tagged P2RX5 is approximately 1ng/ml as a capture antibody.
Applications for P2X5/P2RX5 Antibody (1C5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: P2X5/P2RX5
Long Name
P2X Purinergic Receptor 5
Alternate Names
LRH-1, P2RX5
Entrez Gene IDs
5026 (Human)
Gene Symbol
P2RX5
OMIM
602836 (Human)
UniProt
Additional P2X5/P2RX5 Products
Product Documents for P2X5/P2RX5 Antibody (1C5)
Product Specific Notices for P2X5/P2RX5 Antibody (1C5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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