P2X5/P2RX5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35187
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 341-422 of human P2X5/P2RX5 (NP_002552.2).
Sequence:
KKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST
Sequence:
KKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for P2X5/P2RX5 Antibody
Immunocytochemistry/ Immunofluorescence: P2X5/P2RX5 Antibody [NBP3-35187] -
Immunocytochemistry/ Immunofluorescence: P2X5/P2RX5 Antibody [NBP3-35187] - Immunofluorescence analysis of Jurkat cells using P2X5/P2RX5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: P2X5/P2RX5 Antibody [NBP3-35187] -
Immunocytochemistry/ Immunofluorescence: P2X5/P2RX5 Antibody [NBP3-35187] - Immunofluorescence analysis of paraffin-embedded mouse spleen using P2X5/P2RX5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: P2X5/P2RX5 Antibody [NBP3-35187] -
Western Blot: P2X5/P2RX5 Antibody [NBP3-35187] - Western blot analysis of various lysates using P2X5/P2RX5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Applications for P2X5/P2RX5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: P2X5/P2RX5
Long Name
P2X Purinergic Receptor 5
Alternate Names
LRH-1, P2RX5
Gene Symbol
P2RX5
Additional P2X5/P2RX5 Products
Product Documents for P2X5/P2RX5 Antibody
Product Specific Notices for P2X5/P2RX5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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