P2Y12/P2RY12 Antibody (PSH04-48)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32622
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH04-48
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide. (Uniprot: Q9H244)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for P2Y12/P2RY12 Antibody (PSH04-48)
Immunohistochemistry-Frozen: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622]
Immunohistochemistry-Frozen: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622] - Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-P2Y12/P2RY12 antibody (NBP3-32622) at 1/200 dilution.The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32622, green) at 1/200 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622]
Immunohistochemistry: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-P2Y12/P2RY12 antibody (NBP3-32622) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32622) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for P2Y12/P2RY12 Antibody (PSH04-48)
Application
Recommended Usage
Immunohistochemistry-Frozen
1:200
Immunohistochemistry-Paraffin
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: P2Y12/P2RY12
Long Name
P2Y Purinergic Receptor 12
Alternate Names
ADPG-R, HORK3, P2RY12, SP1999
Gene Symbol
P2RY12
Additional P2Y12/P2RY12 Products
Product Documents for P2Y12/P2RY12 Antibody (PSH04-48)
Product Specific Notices for P2Y12/P2RY12 Antibody (PSH04-48)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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