P2Y12/P2RY12 Antibody

Immunohistochemistry: P2Y12/P2RY12 Antibody [NBP2-33870]

Immunohistochemistry: P2Y12/P2RY12 Antibody [NBP2-33870] - Features of P2Y12/P2RY12-immunoreactive microglia. (I,J). Staining of representative sections with P2Y12/P2RY12 (Novus) antibody preabsorbed with immunizing peptide (I, +Pep) compared to staining of matched section with P2Y12/P2RY12 antibody non-absorbed (J, -Pep). (K,L). Staining of matched sections with alternative P2Y12/P2RY12 antibody. Same staining pattern of microglia revealed with P2Y12/P2RY12 (Novus) antibody (C) as with P2Y12/P2RY12 (Alomone Labs) antibody. All sections shown had been counterstained with neutral red to identify nuclei (red color). Abbreviations: ND: non-demented. AD: Alzheimer's disease. MTG: middle temporal gyrus.-Pep: antibody without immunizing peptide. + Pep: antibody with immunizing peptide. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/21/2/678), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human cerebral cortex shows strong cytoplasmic positivity in microglia.

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human liver shows no positivity as expected.

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human cerebellum shows strong cytoplasmic positivity in microglia.

Immunohistochemistry-Frozen: P2Y12/P2RY12 Antibody [NBP2-33870]

Immunohistochemistry-Frozen: P2Y12/P2RY12 Antibody [NBP2-33870] - Canine optic nerve. Immunofluorescent signal was detected using Alexa Fluor 488 conjugated secondary antibody (green). IHC-Fr image submitted by a verified customer review.

Immunohistochemistry: P2Y12/P2RY12 Antibody [NBP2-33870] -

Immunohistochemistry: P2Y12/P2RY12 Antibody [NBP2-33870] - Images demonstrating colocalization of CD105/MAB1097 with microglial markers (a) panels A, B Low magnification images of high-plaque (HP) case (panel A) & AD case (panel B) showing staining with CD105/MAB1097 (purple arrows) & IBA-1 (brown arrows) in MTG sections. Both images at same magnification: scale bars represent 100 μm. (b) (panel A) CD105 microglia (purple arrows) with ramified morphology stained with antibody to P2RY12 (brown arrow) in low-plaque (LP) case. (panel B) CD105 microglia (purple arrows) & HLA-DR (brown arrow) in AD case. (panel C) CD105 microglia (purple arrow) & CD45 (brown arrow) in AD case. All sections were from MTG. Images at same magnification: scale bars represent 50 μm. (c) (panel A–E). Patterns of staining of CD105 (purple) & IBA-1 (brown) in MTG sections of cases with progressively increasing pathology from LP to AD. (panel F). CD105-positive microglia in hippocampus (HPC) of AD case. Images at same magnification: scale bars represent 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31340569), licensed under a CC-BY license. Not internally tested by Novus Biologicals.