p38 alpha [p Tyr182] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82097

Novus Biologicals, part of Bio-Techne
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NB100-82097
NB100-82097-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all p38 alpha Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human p38 alpha around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).

Modification

p Tyr182

Specificity

Detects endogenous levels of p38 alpha only when phosphorylated at tyrosine 182.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p38 alpha [p Tyr182] Antibody

Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]

Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]

Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.

Immunohistochemistry-Paraffin: p38 alpha [p Tyr182] Antibody [NB100-82097] -

Immunohistochemistry-Paraffin: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82097 (left) or the same antibody preincubated with blocking peptide(right).
Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]

Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]

Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182) antibody.
View 1 more images

Applications for p38 alpha [p Tyr182] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-82097 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p38 alpha

p38 is a 38 kDa Stress Activated Protein Kinase / Map Kinase (SAPK / MAPK) that is fully activated by dual phosphorylation on threonine 180 and tyrosine 182, within the activation loop. p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knockout studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2 and the kinases MAPKAPK2 and MAPKAPK5. p38 MAPK plays a role in the production of IL6 and is thought to stabilize erythropoietin production during hypoxic stress.

Alternate Names

MAPK14, SAPK2a

Entrez Gene IDs

1432 (Human); 26416 (Mouse); 81649 (Rat)

Gene Symbol

MAPK14

UniProt

Q16539

Additional p38 alpha Products

Product Documents for p38 alpha [p Tyr182] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p38 alpha [p Tyr182] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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