p38 gamma/SAPK3 Antibody (OTI10E1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47852
![Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]](https://resources.bio-techne.com/images/products/p38-gamma-SAPK3-Antibody-10E1-Immunohistochemistry-Paraffin-NBP1-47852-img0006.jpg "Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]")
![Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]](https://resources.bio-techne.com/images/products/p38-gamma-SAPK3-Antibody-10E1-Western-Blot-NBP1-47852-img0008.jpg "Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI10E1
Concentration
1 mg/ml
Product Summary for p38 gamma/SAPK3 Antibody (OTI10E1)
Immunogen
Full-length protein expressed in 293T cell transfected with human MAPK12 expression vector
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens mitogen-activated protein kinase 12 (MAPK12).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p38 gamma/SAPK3 Antibody (OTI10E1)
Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]
Western Blot: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - hHEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAPK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAPK3.![Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]](https://resources.bio-techne.com/images/products/p38-gamma-SAPK3-Antibody-10E1-Immunohistochemistry-Paraffin-NBP1-47852-img0007.jpg "Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]")
Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]
Immunohistochemistry-Paraffin: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - Staining of paraffin-embedded Human pancreas tissue using anti-SAPK3 mouse monoclonal antibody.![Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]](https://resources.bio-techne.com/images/products/p38-gamma-SAPK3-Antibody-10E1-Flow-Cytometry-NBP1-47852-img0005.jpg "Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]")
Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) [NBP1-47852]
Flow Cytometry: p38 gamma/SAPK3 Antibody (10E1) [NBP1-47852] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAPK3 antibody, and then analyzed by flow cytometry.Applications for p38 gamma/SAPK3 Antibody (OTI10E1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP1-47852 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: p38 gamma
Additional p38 gamma Products
Product Documents for p38 gamma/SAPK3 Antibody (OTI10E1)
Product Specific Notices for p38 gamma/SAPK3 Antibody (OTI10E1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...