p40/deltaNp63 Antibody (P40/4396R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14050
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # P40/4396R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for p40/deltaNp63 Antibody (P40/4396R) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa1-100) of human p40/deltaNp63 protein (exact sequence is proprietary) (Uniprot: Q9H3D4 )
Localization
Nucleus
Specificity
p40 antibody (antibody recognizing Np63 only) is an isoform of p63, is thought to function as a stem cell factor, responsible for maintaining cells in an uncommitted state with regenerative potential a role that may be recapitulated in tumors derived from these cells. p40 is normally expressed in the basal or progenitor cell layer of stratified epithelia (eg. squamous, urothelial, bronchial), basal cells of some glandular epithelia (eg. prostate), as well as myoepithelial cells of breast and salivary glands, trophoblasts and thymic epithelial cells. In tumor tissues, p40 expression is specific for squamous cell carcinoma. p40 is equivalent to p63 in sensitivity for lung squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. p40 appears to be a more reliable marker for squamous cell carcinoma.
Marker
Squamous, Basal & Myoepithelial Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13805).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Applications for p40/deltaNp63 Antibody (P40/4396R) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: p40/deltaNp63
Alternate Names
AIS, amplified in squamous cell carcinoma, B(p51A), B(p51B), Chronic ulcerative stomatitis protein, EEC3, Keratinocyte transcription factor KET, KET, LMS, OFC8, p53CP, SHFM4, TP53CP, TP53L, TP63, TP73L, Transformation-related protein 63, tumor protein p53-competing protein
Gene Symbol
TP63
Additional p40/deltaNp63 Products
Product Documents for p40/deltaNp63 Antibody (P40/4396R) - Azide and BSA Free
Product Specific Notices for p40/deltaNp63 Antibody (P40/4396R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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