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P4HA1 Antibody (4F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43012

Novus Biologicals, part of Bio-Techne
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NBP3-43012

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4F1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

prolyl 4-hydroxylase, alpha polypeptide I - (Uniprot# P13674)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for P4HA1 Antibody (4F1)

P4HA1 Antibody (4F1)

Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody (4F1) [NBP3-43012] -

Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody (4F1) [NBP3-43012] - Immunofluorescent analysis of (-20℃ Ethanol ) fixed PC-3 cells using (P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
P4HA1 Antibody (4F1)

Western Blot: P4HA1 Antibody (4F1) [NBP3-43012] -

Western Blot: P4HA1 Antibody (4F1) [NBP3-43012] - PC-3 cells were subjected to SDS PAGE followed by western blot with (P4HA1 antibody) at dilution of 1:1000
P4HA1 Antibody (4F1)

Immunohistochemistry: P4HA1 Antibody (4F1) [NBP3-43012] -

Immunohistochemistry: P4HA1 Antibody (4F1) [NBP3-43012] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (P4HA1 Antibody) at dilution of 1:500 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Applications for P4HA1 Antibody (4F1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: P4HA1

P4HA1 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Long Name

Prolyl 4-Hydroxylase Subunit Alpha 1

Alternate Names

4-PH Alpha-1, P4HA

Gene Symbol

P4HA1

Additional P4HA1 Products

Product Documents for P4HA1 Antibody (4F1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for P4HA1 Antibody (4F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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