P4HA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57852

Novus Biologicals, part of Bio-Techne
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NB100-57852
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all P4HA1 Antibodies »

Product Specifications

Immunogen

This P4HA1 Antibody was developed against a peptide with sequence C-DFARKDEPDAFKE corresponding to internal region according to NP_001017962.1.

Epitope

DFARKDEPDAFKE

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24993042).

Specificity

This antibody is expected to recognize both reported isoforms (NP_000908.2 and NP_001017962.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for P4HA1 Antibody

Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody [NB100-57852]

Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody [NB100-57852]

Immunocytochemistry/Immunofluorescence: P4HA1 Antibody [NB100-57852] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852]

Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Staining of paraffin embedded Human Spleen with antibody at 7 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for P4HA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

7 ug/mL

Immunohistochemistry-Paraffin

7 ug/mL

Peptide ELISA

Detection limit 1:128000
Application Notes
IHC-P usage reported in scientific literature (PMID: 25587023).

Reviewed Applications

Read 1 review rated 5 using NB100-57852 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: P4HA1

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Long Name

Prolyl 4-Hydroxylase Subunit Alpha 1

Alternate Names

4-PH Alpha-1, P4HA

Entrez Gene IDs

5033 (Human); 18451 (Mouse); 64475 (Rat)

Gene Symbol

P4HA1

UniProt

P13674 (Human)

Additional P4HA1 Products

Product Documents for P4HA1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for P4HA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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