P4HA2 Antibody (CL0351)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52922

Novus Biologicals, part of Bio-Techne
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NBP2-52922
NBP2-52922-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (90%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0351

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all P4HA2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGM

Epitope

NTDWPALEDLVLQDS

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for P4HA2 Antibody (CL0351)

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922]

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922]

Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.
Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922]

Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922]

Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-P4HA2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922]

Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922]

Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
View 4 more images

Applications for P4HA2 Antibody (CL0351)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P4HA2

P4HA2 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

2-oxoglutarate-4-dioxygenase subunit alpha-2, 4-PH alpha 2, 4-PH alpha-2, alpha polypeptide II, collagen prolyl 4-hydroxylase alpha(II), C-P4Halpha(II), EC 1.14.11.2, Procollagen-proline, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), prolyl 4-hydroxylase subunit alpha-2, prolyl 4-hydroxylase, alpha polypeptide II

Gene Symbol

P4HA2

Additional P4HA2 Products

Product Documents for P4HA2 Antibody (CL0351)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for P4HA2 Antibody (CL0351)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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