Key Product Details

Species Reactivity

Validated:

Human, Canine, Chicken, Hamster, Monkey, Mouse (Negative), Rat (Negative)

Cited:

Human, Mouse, Avian - Chicken, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Block/Neutralize, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # BP53-12

Concentration

0.2 mg/ml

View all p53 Antibodies »

Product Specifications

Immunogen

Recombinant human wild-type p53 protein (Uniprot: P04637)

Epitope

aa 16-25

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Predominantly nuclear

Specificity

This monoclonal antibody reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33074)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for p53 Antibody (BP53-12)

Western Blot: p53 Antibody (BP53-12) [NBP2-29453]

Western Blot: p53 Antibody (BP53-12) [NBP2-29453]

Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).
Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453]

Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453]

Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).
Western Blot: p53 Antibody (BP53-12) [NBP2-29453]

Western Blot: p53 Antibody (BP53-12) [NBP2-29453]

Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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Applications for p53 Antibody (BP53-12)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.

Reviewed Applications

Read 1 review rated 5 using NBP2-29453 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p53

Nuclear protein p53 plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutations in the evolutionarily conserved codons of the p53 tumor suppressor gene are common in diverse types of human cancer, which includes cancer of colon, lung, esophagus, breast, liver, brain, reticuloendothelial tissues, and hemopoietic tissues. p53 maps to the 17p13.1 region of the human chromosome. Consistent with its role as a tumor suppressor gene in a wide variety of tissue types, p53 performs several different critical functions in regulating cellular growth, replication, and death. p53 positively regulates transcription by binding to specific DNA consensus sequences. These sequences are associated with several known genes including the human ribosomal gene cluster, muscle creatine kinase gene, WAF-1/CIPI/p21, and cyclin G. This sequence-specific transcriptional activation is associated with the induction of growth suppression. p53 also negatively regulates transcription of genes which have TATA box initiated promoters, likely by binding to protein components of the basal transcription machinery. Possibly through the general mechanism of transcriptional regulation, p53 affects cell behavior via several specific pathways. Wild-type p53 suppresses cell proliferation by blocking the transition from G1 to S-phase. This may occur through p53 mediated induction of a universal inhibitor of cyclin-dependent kinases, WAF-1 or CIPI. Increased expression of WAF 1/CIPI/p21 serves to inhibit phosphorylation of Rb protein by cdk complexes, resulting in failure to progress from G1 to S phase. This p53 antibody recognizes a 53kDa protein, which is identified as p53 suppressor gene product. The antibody reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 Antibody (BP53-12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p53 Antibody (BP53-12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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