p53 Antibody (OTI5E2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73200

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5E2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all p53 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p53 Antibody (OTI5E2) - Azide and BSA Free

Western Blot: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]

Western Blot: p53 Antibody (OTI5E2)Azide and BSA Free [NBP2-73200]

Western Blot: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.
Immunocytochemistry/ Immunofluorescence: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]

Immunocytochemistry/ Immunofluorescence: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]

Immunocytochemistry/Immunofluorescence: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.
Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]

Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200]

Immunohistochemistry: p53 Antibody (OTI5E2) - Azide and BSA Free [NBP2-73200] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.
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Applications for p53 Antibody (OTI5E2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 Antibody (OTI5E2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p53 Antibody (OTI5E2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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