Key Product Details

Species Reactivity

Zebrafish

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V5P4H6*B3

Format

BSA Free

Concentration

1 mg/ml

View all p53 Antibodies »

Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptide KLMVKDEGRS (C-terminal sequence)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for p53 Antibody (V5P4H6*B3) - BSA Free

Immunohistochemistry: p53 Antibody (V5P4H6*B3) - BSA Free [NBP2-50231]

Immunohistochemistry: p53 Antibody (V5P4H6*B3) - BSA Free [NBP2-50231]

Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.

Applications for p53 Antibody (V5P4H6*B3) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): WB - whole Zebrafish lysate; IHC- Zebrafish embryo.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 Antibody (V5P4H6*B3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for p53 Antibody (V5P4H6*B3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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