p53 [p Ser392] Antibody - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NB200-156

Novus Biologicals, part of Bio-Techne
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NB200-156
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p53 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392 conjugated to KLH. Accession # P04637

Modification

p Ser392

Specificity

Specific for endogenous levels of the ~53 kDa p53 protein phosphorylated at Ser392 ~53 kDa p53 protein phosphorylated at Ser392. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p53 [p Ser392] Antibody - Azide Free

Western Blot: p53 [p Ser392] Antibody [NB200-156]

Western Blot: p53 [p Ser392] Antibody [NB200-156]

Western Blot: p53 [p Ser392] Antibody [NB200-156] - rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k p53 phosphorylated Rat Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for p53 [p Ser392] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
NB 200-156 can be used in Western blot where a band is seen at ~ 53 kDa, representing p53.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p53

p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas. Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene. The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression. p53's role in the multi-step process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human)

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 [p Ser392] Antibody - Azide Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p53 [p Ser392] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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