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p57 Kip2 Antibody (6T4E6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16648

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16648-100ul
NBP3-16648-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6T4E6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for p57 Kip2 Antibody (6T4E6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p57 Kip2 (P49918). MSDASLRSTSTMERLVARGTFPVLVRTSACRSLFGPVDHEELSRELQARLAELNAEDQNRWDYDFQQDMPLRGPGRLQWTEVDSDSVPAFYRETVQVGRC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p57 Kip2 Antibody (6T4E6)

Western Blot: p57 Kip2 Antibody (6T4E6) [NBP3-16648]

Western Blot: p57 Kip2 Antibody (6T4E6) [NBP3-16648]

Western Blot: p57 Kip2 Antibody (6T4E6) [NBP3-16648] - Analysis of extracts of various cell lines, using p57 Kip2 Rabbit mAb (NBP3-16648) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for p57 Kip2 Antibody (6T4E6)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p57 Kip2

p57kip2 is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate.

Alternate Names

Beckwith-Wiedemann syndrome, BWS, cyclin-dependent kinase inhibitor 1C, cyclin-dependent kinase inhibitor 1C (p57, Kip2), Cyclin-dependent kinase inhibitor p57, KIP2BWCR, p57, p57Kip2, WBS

Gene Symbol

CDKN1C

Additional p57 Kip2 Products

Product Documents for p57 Kip2 Antibody (6T4E6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p57 Kip2 Antibody (6T4E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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