Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all p57 Kip2 Antibodies »

Product Summary for p57 Kip2 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 266 and 316 of human cyclin-dependent kinase inhibitor p57 using the numbering given in entry NP_000067.1 (GeneID 1028).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p57 Kip2 Antibody

Immunoprecipitation: p57 Kip2 Antibody [NBP1-28707]

Immunoprecipitation: p57 Kip2 Antibody [NBP1-28707]

Immunoprecipitation: p57 Kip2 Antibody [NBP1-28707] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p57kip2 antibody NBP1-28706 used for IP at 3 ug/mg lysate. p57kip2 was also immunoprecipitated by rabbit anti-p57kip2 antibody NBP1-28707, which recognizes an upstream epitope. For blotting immunoprecipitated p57kip2, NBP1-28706 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for p57 Kip2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
This antibody is useful for immunoprecipitation. It is not recommended for western analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p57 Kip2

p57kip2 is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate.

Alternate Names

Beckwith-Wiedemann syndrome, BWS, cyclin-dependent kinase inhibitor 1C, cyclin-dependent kinase inhibitor 1C (p57, Kip2), Cyclin-dependent kinase inhibitor p57, KIP2BWCR, p57, p57Kip2, WBS

Entrez Gene IDs

1028 (Human)

Gene Symbol

CDKN1C

UniProt

NP_000067.1 (Human)

Additional p57 Kip2 Products

Product Documents for p57 Kip2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p57 Kip2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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