p66 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87359

Novus Biologicals, part of Bio-Techne
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NBP1-87359
NBP1-87359-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p66 alpha Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RRKLAFRSGEARDWSNGAVLQASSQLSRGSATTPRGVLHTFSPSPKLQNSASATALVSRTGRHSERTVSAGKGSATSNWKKTPLSTGGTLAFVSPSLAVHKSSSAVDRQREYLLDMIPPRSIP

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25190814).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p66 alpha Antibody

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87359]

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87359]

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87359] - Staining of human colon, placenta, skeletal muscle and testis using Anti-p66 alpha antibody NBP1-87359 (A) shows similar protein distribution across tissues to independent antibody NBP1-87360 (B).
Western Blot: p66 alpha Antibody [NBP1-87359]

Western Blot: p66 alpha Antibody [NBP1-87359]

Western Blot: p66 alpha Antibody [NBP1-87359] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: p66 alpha Antibody [NBP1-87359]

Immunocytochemistry/ Immunofluorescence: p66 alpha Antibody [NBP1-87359]

Immunocytochemistry/Immunofluorescence: p66 alpha Antibody [NBP1-87359] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
View 5 more images

Applications for p66 alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
IP, WB reported in scientific literature (PMID: 25190814). Use in CHIP reported in scientific literature (PMID:31934863). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p66 alpha

Methylation of cytosine at CpG dinucleotides is a common feature of many higher eukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histone deacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism of methylated DNA silencing. P66, a zinc finger-containing protein is one of the components of the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different forms of the same zinc finger-containing protein and are conserved from C.elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 and CR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci (4).

Alternate Names

FLJ20085, FLJ21017, GATA zinc finger domain containing 2A, GATA zinc finger domain-containing protein 2A, hp66alpha, p66 alpha, p66alpha, transcriptional repressor p66-alpha

Entrez Gene IDs

54815 (Human)

Gene Symbol

GATAD2A

UniProt

Q86YP4 (Human)

Additional p66 alpha Products

Product Documents for p66 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p66 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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