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p70 S6 Kinase beta/S6K2 Antibody (4B11)

Novus Biologicals, part of Bio-Techne | Catalog # H00006199-M08

Novus Biologicals, part of Bio-Techne
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H00006199-M08

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RPS6KB2 (AAH06106, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHSFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKV

Specificity

RPS6KB2 (4B11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for p70 S6 Kinase beta/S6K2 Antibody (4B11)

Western Blot: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08]

Western Blot: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08]

Western Blot: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Analysis of RPS6KB2 expression in transfected 293T cell line by RPS6KB2 monoclonal antibody (M08), clone 4B11. Lane 1: RPS6KB2 transfected lysatE (53.4 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08]

Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08]

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Analysis of monoclonal antibody to RPS6KB2 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08]

ELISA: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08]

ELISA: p70 S6 Kinase beta/S6K2 Antibody (4B11) [H00006199-M08] - Detection limit for recombinant GST tagged RPS6KB2 is approximately 0.1ng/ml as a capture antibody.

Applications for p70 S6 Kinase beta/S6K2 Antibody (4B11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p70 S6 Kinase beta

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.

Alternate Names

KLS, p70-beta, RPS6KB2, STK14B

Entrez Gene IDs

6199 (Human)

Gene Symbol

RPS6KB2

OMIM

608939 (Human)

Additional p70 S6 Kinase beta Products

Product Documents for p70 S6 Kinase beta/S6K2 Antibody (4B11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p70 S6 Kinase beta/S6K2 Antibody (4B11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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