p70 S6 Kinase beta/S6K2 Antibody (5D8F5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16752

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16752-100ul
NBP3-16752-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5D8F5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p70 S6 Kinase beta Antibodies »

Product Summary for p70 S6 Kinase beta/S6K2 Antibody (5D8F5)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human p70 S6 Kinase beta/S6K2 (Q9UBS0). MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHCFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDTAHTRAER

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p70 S6 Kinase beta/S6K2 Antibody (5D8F5)

Western Blot: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752]

Western Blot: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752]

Western Blot: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Western blot analysis of extracts of Mouse brain, using p70 S6 Kinase beta/S6K2 Rabbit mAb (NBP3-16752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752]

Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752]

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunofluorescence analysis of U-2 OS cells using p70 S6 Kinase beta/S6K2 Rabbit mAb (NBP3-16752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752]

Immunohistochemistry-Paraffin: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752]

Immunohistochemistry-Paraffin: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Immunohistochemistry of paraffin-embedded mouse kidney using p70 S6 Kinase beta/S6K2 Rabbit mAb (NBP3-16752) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for p70 S6 Kinase beta/S6K2 Antibody (5D8F5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p70 S6 Kinase beta

S6K2 encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.

Alternate Names

KLS, p70-beta, RPS6KB2, STK14B

Gene Symbol

RPS6KB2

Additional p70 S6 Kinase beta Products

Product Documents for p70 S6 Kinase beta/S6K2 Antibody (5D8F5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p70 S6 Kinase beta/S6K2 Antibody (5D8F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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