p70 S6 Kinase/S6K Antibody (4H4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006198-M04

Novus Biologicals, part of Bio-Techne
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H00006198-M04
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4H4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p70 S6 Kinase Antibodies »

Product Specifications

Immunogen

RPS6KB1 (NP_003152, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for p70 S6 Kinase/S6K Antibody (4H4)

Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04]

Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04]

Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of RPS6KB1 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04]

Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04]

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of monoclonal antibody to RPS6KB1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04]

Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04]

Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - s6k in fibroblasts. Image from verified customer review.
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Applications for p70 S6 Kinase/S6K Antibody (4H4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
It has been used for ELISA, IF and WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using H00006198-M04 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p70 S6 Kinase

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Entrez Gene IDs

6198 (Human)

Gene Symbol

RPS6KB1

OMIM

608938 (Human)

UniProt

NP_003152 (Human)

Additional p70 S6 Kinase Products

Product Documents for p70 S6 Kinase/S6K Antibody (4H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p70 S6 Kinase/S6K Antibody (4H4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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