p70 S6 Kinase/S6K Antibody (OTI1G4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00754
![Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]](https://resources.bio-techne.com/images/products/S6K-Antibody-1G4-Flow-Cytometry-NBP2-00754-img0002.jpg "Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1G4
Concentration
0.71 mg/ml
Product Summary for p70 S6 Kinase/S6K Antibody (OTI1G4)
Immunogen
Full length human recombinant protein of human RPS6KB1(NP_003152) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p70 S6 Kinase/S6K Antibody (OTI1G4)
Western Blot: S6K Antibody (1G4) [NBP2-00754] Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]](https://resources.bio-techne.com/images/products/S6K-Antibody-1G4-Immunocytochemistry-Immunofluorescence-NBP2-00754-img0007.jpg "Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]")
Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]
Immunocytochemistry/Immunofluorescence: S6K Antibody (1G4) [NBP2-00754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY S6K.![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]](https://resources.bio-techne.com/images/products/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0006.jpg "Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]")
Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]
Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Human pancreas tissue using anti-S6K mouse monoclonal antibody.![Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]](https://resources.bio-techne.com/images/products/S6K-Antibody-1G4-Western-Blot-NBP2-00754-img0003.jpg "Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]")
![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]](https://resources.bio-techne.com/images/products/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0004.jpg "Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]")
![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]](https://resources.bio-techne.com/images/products/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0005.jpg "Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]")
![Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]](https://resources.bio-techne.com/images/products/S6K-Antibody-1G4-Flow-Cytometry-NBP2-00754-img0001.jpg "Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) [NBP2-00754]")
Applications for p70 S6 Kinase/S6K Antibody (OTI1G4)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.71 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: p70 S6 Kinase
Additional p70 S6 Kinase Products
Product Documents for p70 S6 Kinase/S6K Antibody (OTI1G4)
Product Specific Notices for p70 S6 Kinase/S6K Antibody (OTI1G4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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