Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all p70 S6 Kinase Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 475 and the C-terminus (residue 525) of human Ribosomal Protein S6 Kinase, 70 kDa, polypeptide 1 using the numbering given in entry NP_003152.1 (GeneID 6198).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p70 S6 Kinase/S6K Antibody

Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293]

Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293]

Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for p70 S6 Kinase/S6K Antibody

Application
Recommended Usage

Western Blot

1:2500-1:20000
Application Notes
Useful for Western blot where a 70kDa band is seen. *The investigator should determine the optimal working dilution for a specific application.
See Simple Western Antibody Database for Simple Western validation: tested in treated and untreated MCF7; antibody dilution of 1:50; separated by size

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p70 S6 Kinase

Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka) and S6K2 (S6Kb). The S6K1 isoform has two splicing forms, which are the products of alternative start codon usage. The first splicing form, p70S6K or p70S6K1, has a predicted molecular mass of 56 kDa and an apparent molecular mass (SDS-PAGE) of 70 kDa. The second isoform, p85S6K or p85S6K1, contains 23 extra amino acid residues at the N terminus, resulting from the alternative start codon. It has a predicted molecular mass of 59 kDa and an apparent molecular mass of 85 kDa. Both, p70 and p85 S6K1 are activated by multiple S/T phosphorylations in response to various mitogenic stimuli and nutrients. This is accompanied by significant mobility shift in the migration of both forms, when analysed by SDS-PAGE electrophoresis.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Entrez Gene IDs

6198 (Human); 83840 (Rat)

Gene Symbol

RPS6KB1

UniProt

NP_003152.1 (Human)

Additional p70 S6 Kinase Products

Product Documents for p70 S6 Kinase/S6K Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p70 S6 Kinase/S6K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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