p97/VCP Antibody (3P8T3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15478

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15478-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3P8T3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VCP Antibodies »

Product Summary for p97/VCP Antibody (3P8T3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p97/VCP (P55072). MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p97/VCP Antibody (3P8T3)

Western Blot: p97/VCP Antibody (3P8T3) [NBP3-15478]

Western Blot: p97/VCP Antibody (3P8T3) [NBP3-15478]

Western Blot: p97/VCP Antibody (3P8T3) [NBP3-15478] - Western blot analysis of extracts of various cell lines, using p97/VCP Rabbit mAb (NBP3-15478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
p97/VCP Antibody (3P8T3)

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] -

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] - Confocal imaging of U-2 OS cells using VCP Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
p97/VCP Antibody (3P8T3)

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] -

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] -Confocal imaging of PC-12 cells using VCP Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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Applications for p97/VCP Antibody (3P8T3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:200 - 1:800

Western Blot

1:1000 - 1:6000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VCP

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Gene Symbol

VCP

Additional VCP Products

Product Documents for p97/VCP Antibody (3P8T3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p97/VCP Antibody (3P8T3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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