p97/VCP Antibody (4G9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-39850
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4G9
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Recombinant human p97/VCP.
Specificity
Recombinant human p97/VCP protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for p97/VCP Antibody (4G9) - BSA Free
![Western Blot: p97/VCP Antibody (4G9)BSA Free [NBP1-39850]](https://resources.bio-techne.com/images/products/p97-VCP-Antibody-4G9-BSA-Free-Western-Blot-NBP1-39850-img0003.jpg "Western Blot: p97/VCP Antibody (4G9)BSA Free [NBP1-39850]")
Western Blot: p97/VCP Antibody (4G9)BSA Free [NBP1-39850]
Western Blot: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9![Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]](https://resources.bio-techne.com/images/products/p97-VCP-Antibody-4G9-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-39850-img0004.jpg "Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]")
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]
Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI.![Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]](https://resources.bio-techne.com/images/products/p97-VCP-Antibody-4G9-BSA-Free-Immunohistochemistry-Paraffin-NBP1-39850-img0001.jpg "Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]")
Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850]
Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - Analysis of anti-VCP antibody with human prostate at concentration 5 ug/ml.Applications for p97/VCP Antibody (4G9) - BSA Free
Application
Recommended Usage
Immunofluorescence
1-4 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
IHC: NBP1-39850 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2). Sourced from tissue culture supernatant.
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: VCP
Long Name
Valosin-containing Protein
Alternate Names
IBMPFD, TERA
Entrez Gene IDs
7415 (Human)
Gene Symbol
VCP
UniProt
Additional VCP Products
Product Documents for p97/VCP Antibody (4G9) - BSA Free
Product Specific Notices for p97/VCP Antibody (4G9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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