PACE4/PCSK6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05030

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PACE4/PCSK6 Antibodies »

Product Specifications

Immunogen

Peptide with sequence PDCEPGTYFDSE corresponding to internal region according to NP_612192.1, NP_612195.1, NP_612197.1, NP_612196.1, NP_612198.2, NP_612193.1, NP_612194.1.

Epitope

internal region

Reactivity Notes

Expected from sequence similarity: Mouse and Rat

Specificity

This antibody is expected to recognize reported isoforms a, b, g, h (NP_002561.1; NP_612192.1; NP_612193.1; NP_612194.1 resp.).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PACE4/PCSK6 Antibody

Immunohistochemistry-Paraffin: PACE4/PCSK6 Antibody [NBP1-05030]

Immunohistochemistry-Paraffin: PACE4/PCSK6 Antibody [NBP1-05030]

Immunohistochemistry-Paraffin: PACE4 Antibody [NBP1-05030] - (3.75ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PACE4/PCSK6 Antibody [NBP1-05030]

Immunohistochemistry-Paraffin: PACE4/PCSK6 Antibody [NBP1-05030]

Immunohistochemistry-Paraffin: PACE4 Antibody [NBP1-05030] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PACE4/PCSK6 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx. 70kDa band in Human, Mouse and Rat Liver lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 105 to 107kDa according to NP_002561.1, NP_612192.1, NP_612193.1 and NP_612194.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Testis and Skeletal Muscle.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PACE4/PCSK6

The protein encoded by the PACE4 gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. Some of its substrates are - transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression. Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Proprotein Convertase Subtilisin/Kexin Type 6

Alternate Names

PACE4, PCSK6, Proprotein Convertase 6, SPC4

Entrez Gene IDs

5046 (Human); 18553 (Mouse); 25507 (Rat)

Gene Symbol

PCSK6

UniProt

NP_002561.1 (Human)

Additional PACE4/PCSK6 Products

Product Documents for PACE4/PCSK6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PACE4/PCSK6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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