PACSIN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33756

Novus Biologicals, part of Bio-Techne
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NBP2-33756
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PACSIN1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EKQPKKAEGVALTNATGAVESTSQAGDRGSVSSYDRGQPYATEWSDDESGN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PACSIN1 Antibody

Western Blot: PACSIN1 Antibody [NBP2-33756]

Western Blot: PACSIN1 Antibody [NBP2-33756]

Western Blot: PACSIN1 Antibody [NBP2-33756] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10, Lane 2: Human Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: PACSIN1 Antibody [NBP2-33756]

Immunocytochemistry/ Immunofluorescence: PACSIN1 Antibody [NBP2-33756]

Immunocytochemistry/Immunofluorescence: PACSIN1 Antibody [NBP2-33756] - Staining of human cell line MCF7 shows localization to nucleus, nucleoli & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PACSIN1 Antibody [NBP2-33756]

Immunohistochemistry-Paraffin: PACSIN1 Antibody [NBP2-33756]

Immunohistochemistry-Paraffin: PACSIN1 Antibody [NBP2-33756] - Staining of human skeletal muscle shows low expression as expected.
View 2 more images

Applications for PACSIN1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

reported by internal validation

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western internal validation: Human Cortex lysate as sample; separated by size; antibody dilution of 1:25; observed molecular weight was 65 kDa; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PACSIN1

PACSINs are a family of cytoplasmic phosphoproteins that play a role in vesicle formation and transport. Highly expressed in brain and to a lesser extent in heart, pancreas and liver.

Alternate Names

KIAA1379SDPI, protein kinase C and casein kinase substrate in neurons 1, protein kinase C and casein kinase substrate in neurons protein 1, syndapin I

Gene Symbol

PACSIN1

UniProt

Q9BY11 (Human)

Additional PACSIN1 Products

Product Documents for PACSIN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PACSIN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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