PACSIN2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06469
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human PACSIN2 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PACSIN2 Antibody
Western Blot: PACSIN2 Antibody [NBP3-06469]
Western Blot: PACSIN2 Antibody [NBP3-06469] - Lane A: Jurkat Whole Cell LysateLane B: NIH/3T3 Whole Cell LysateLane C: RAW 264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:62 kDa(We are unsure as to the identity of these extra bands.)Immunohistochemistry-Paraffin: PACSIN2 Antibody [NBP3-06469]
Immunohistochemistry-Paraffin: PACSIN2 Antibody [NBP3-06469] - Staining of human PACSIN2 in human kidney with rabbit polyclonal antibody at 1:100 dilution.Immunoprecipitation: PACSIN2 Antibody [NBP3-06469]
Immunoprecipitation: PACSIN2 Antibody [NBP3-06469] - Lane A:0.5 mg NIH/3T3 Whole Cell Lysate4 uL anti-PACSIN2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PACSIN2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 56 kDaObserved band size :60 kDaApplications for PACSIN2 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PACSIN2
Alternate Names
cytoplasmic phosphoprotein PACSIN2, protein kinase C and casein kinase substrate in neurons 2, protein kinase C and casein kinase substrate in neurons protein 2, SDPII, syndapin II
Gene Symbol
PACSIN2
Additional PACSIN2 Products
Product Specific Notices for PACSIN2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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