PACT Antibody (2W2E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16701

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16701-20ul
NBP3-16701-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2W2E10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PACT Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 214-313 of human PACT (O75569). TWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PACT Antibody (2W2E10)

Western Blot: PACT Antibody (2W2E10) [NBP3-16701]

Western Blot: PACT Antibody (2W2E10) [NBP3-16701]

Western Blot: PACT Antibody (2W2E10) [NBP3-16701] - Western blot analysis of extracts of various cell lines, using PACTRabbit mAb (NBP3-16701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: PACT Antibody (2W2E10) [NBP3-16701]

Immunohistochemistry-Paraffin: PACT Antibody (2W2E10) [NBP3-16701]

Immunohistochemistry-Paraffin: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry of paraffin-embedded mouse spinal cord using PACTRabbit mAb (NBP3-16701) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PACT Antibody (2W2E10) [NBP3-16701]

Immunohistochemistry-Paraffin: PACT Antibody (2W2E10) [NBP3-16701]

Immunohistochemistry-Paraffin: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry of paraffin-embedded rat brain using PACTRabbit mAb (NBP3-16701) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for PACT Antibody (2W2E10)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PACT

PACT encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Alternate Names

DYT16, HSD14, PKR-Associating Protein X, PRKRA, RAX

Gene Symbol

PRKRA

Additional PACT Products

Product Documents for PACT Antibody (2W2E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PACT Antibody (2W2E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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