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PACT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55124

Novus Biologicals, part of Bio-Techne
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NBP2-55124
NBP2-55124-25ul

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PACT Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: KLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PACT Antibody

Western Blot: PACT Antibody [NBP2-55124]

Western Blot: PACT Antibody [NBP2-55124]

Western Blot: PACT Antibody [NBP2-55124] - Analysis using Anti-PRKRA antibody NBP2-55124 (A) shows similar pattern to independent antibody NBP2-55123 (B).
Immunocytochemistry/ Immunofluorescence: PACT Antibody [NBP2-55124]

Immunocytochemistry/ Immunofluorescence: PACT Antibody [NBP2-55124]

Immunocytochemistry/Immunofluorescence: PACT Antibody [NBP2-55124] - Staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: PACT Antibody [NBP2-55124]

Immunohistochemistry-Paraffin: PACT Antibody [NBP2-55124]

Immunohistochemistry-Paraffin: PACT Antibody [NBP2-55124] - Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.

Applications for PACT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PACT

PACT encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Alternate Names

DYT16, HSD14, PKR-Associating Protein X, PRKRA, RAX

Gene Symbol

PRKRA

Additional PACT Products

Product Documents for PACT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PACT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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