PADI4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97309

Novus Biologicals, part of Bio-Techne
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NBP1-97309
NBP1-97309SS
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Key Product Details

Species Reactivity

Mouse

Applications

ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

2.2 mg/ml

View all PADI4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PAD4 protein (between residues 200-250) [UniProt Q9UM07]

Localization

Cytoplasm. Nucleus. Cytoplasmic granule. Note: Cytoplasmic granules of eosinophils and neutrophils.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PADI4 Antibody - BSA Free

Immunohistochemistry: PADI4 Antibody - BSA Free [NBP1-97309]

Immunohistochemistry: PADI4 Antibody - BSA Free [NBP1-97309]

Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.

Applications for PADI4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:400
Application Notes
This PAD4 antibody is useful for IHC-paraffin embedded sections where staining was observed in the nucleus and cytoplasm of mouse liver tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot or Immunocytochemistry.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

2.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PADI4

Protein-arginine deiminase type-4 (PAD4) is a member of the protein arginine deiminase family (also known as peptidylarginine deiminase IV, PAD, PDI4, PDI5, and PADI5). Enzymes in the protein arginine deiminase family catalyze the conversion of arginine residues into citrulline residues. More specifically, PAD4 converts arginine residues to citrulline at Arg-8 and Arg-17 on histone H3 and Arg-3 on histone H4, thus preventing methylation at these sites by CARM1 and HRMT1L2/PRMT1. PAD4 also induces a favorable interaction between EP300/P300 and NCOA2/GRIP1 by citrullinating EP300/P300 at Arg-2142. In addition, the PAD4 enzyme may also be involved in the development of granulocytes and macrophages. PAD4 is expressed in the cytoplasmic granules of eosinophils and neutrophils, and is not expressed in peripheral monocytes or lymphocytes. Areas of interest involving PAD4 research include inflammation, immune response, and rheumatoid arthritis.

Long Name

Peptidyl Arginine Deiminase type IV

Alternate Names

HL-60 PAD, PAD, PAD4, PDI4

Entrez Gene IDs

23569 (Human)

Gene Symbol

PADI4

UniProt

Q9UM07 (Human)

Additional PADI4 Products

Product Documents for PADI4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PADI4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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