Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PAK4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4. The immunogen is located within amino acids 210 - 260 of PAK4. Amino Acid Squence: GPSAGGLAIPQS

Specificity

PAK4 antibody is predicted to not cross-react with other PAK family proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAK4 Antibody - BSA Free

Western Blot: PAK4 AntibodyBSA Free [NBP1-77236]

Western Blot: PAK4 AntibodyBSA Free [NBP1-77236]

Western Blot: PAK4 Antibody [NBP1-77236] - SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 u/mL.
PAK4 Antibody - BSA Free

Immunohistochemistry: PAK4 Antibody - BSA Free [NBP1-77236] -

Immunohistochemistry: PAK4 Antibody - BSA Free [NBP1-77236] - Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 u/mL.
PAK4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PAK4 Antibody - BSA Free [NBP1-77236] -

Immunocytochemistry/ Immunofluorescence: PAK4 Antibody - BSA Free [NBP1-77236] - Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 ug/mL.

Applications for PAK4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAK4

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

Long Name

p21/Cdc42/Rac1-activated Kinase 4

Alternate Names

EC 2.7.11, EC 2.7.11.1, KIAA1142, p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4

Gene Symbol

PAK4

UniProt

NP_005875 (Human)

Additional PAK4 Products

Product Documents for PAK4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PAK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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