PAK4 Antibody [mFluor Violet 500 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77236MFV500

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-77236AF350

Alexa Fluor 405

NBP1-77236AF405

Alexa Fluor 488

NBP1-77236AF488

Alexa Fluor 532

NBP1-77236AF532

Alexa Fluor 594

NBP1-77236AF594

Alexa Fluor 647

NBP1-77236AF647

Alexa Fluor 700

NBP1-77236AF700

Alexa Fluor 750

NBP1-77236AF750

Biotin

NBP1-77236B

CoraFluor 1

NBP1-77236CL1

DyLight 350

NBP1-77236UV

DyLight 405

NBP1-77236V

DyLight 488

NBP1-77236G

DyLight 550

NBP1-77236R

DyLight 594

NBP1-77236DL594

DyLight 650

NBP1-77236C

DyLight 680

NBP1-77236FR

DyLight 755

NBP1-77236IR

FITC

NBP1-77236F

HRP

NBP1-77236H

Janelia Fluor 525

NBP1-77236JF525

Janelia Fluor 549

NBP1-77236JF549

Janelia Fluor 585

NBP1-77236JF585

Janelia Fluor 635

NBP1-77236JF635

Janelia Fluor 646

NBP1-77236JF646

Janelia Fluor 669

NBP1-77236JF669

mFluor Violet 450 SE

NBP1-77236MFV450

mFluor Violet 610 SE

NBP1-77236MFV610

PerCP

NBP1-77236PCP

Forumulation

Catalog #

BSA Free

NBP1-77236

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAK4 Antibodies »

Product Summary for PAK4 Antibody [mFluor Violet 500 SE]

Immunogen

Antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4. The immunogen is located within amino acids 210 - 260 of PAK4. Amino Acid Squence: GPSAGGLAIPQS

Specificity

PAK4 antibody is predicted to not cross-react with other PAK family proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for PAK4 Antibody [mFluor Violet 500 SE]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: PAK4

p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific antibodies directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins.

Long Name

p21/Cdc42/Rac1-activated Kinase 4

Alternate Names

EC 2.7.11, EC 2.7.11.1, KIAA1142, p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4

Gene Symbol

PAK4

Additional PAK4 Products

Product Documents for PAK4 Antibody [mFluor Violet 500 SE]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for PAK4 Antibody [mFluor Violet 500 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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