Palladin Antibody (JE66-56)

Western Blot: Palladin Antibody (JE66-56) [NBP3-32709]

Western Blot: Palladin Antibody (JE66-56) [NBP3-32709] - Western blot analysis of Palladin on different lysates with Rabbit anti-Palladin antibody (NBP3-32709) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15ug/Lane)
Lane 2: SiHa cell lysate (10ug/Lane)
Lane 3: A431 cell lysate (15 ug/Lane)
Lane 4: SW480 cell lysate (30 ug/Lane)
Lane 5: 293T cell lysate (20 ug/Lane)
Lane 6: HEK-293 cell lysate (20 ug/Lane)
Lane 7: MDA-MB-231 cell lysate (20 ug/Lane)
Lane 8: MDA-MB-468 cell lysate (20 ug/Lane)
Lane 9: MCF7 cell lysate (20 ug/Lane)

Predicted band size: 151 kDa
Observed band size: 110/151 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32709) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.