pan Cadherin Antibody (0J8Q6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05342

Novus Biologicals, part of Bio-Techne
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NBP3-05342-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 0J8Q6

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cadherin Antibodies »

Product Summary for pan Cadherin Antibody (0J8Q6) - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 373-622 of human pan Cadherin (P12830). PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPN

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for pan Cadherin Antibody (0J8Q6) - Azide and BSA Free

pan Cadherin Antibody (2M6G8)

Western Blot: pan Cadherin Antibody (2M6G8) [NBP3-05342] -

Western Blot: pan Cadherin Antibody (2M6G8) [NBP3-05342] - analysis of extracts of various cell lines, using E-Cadherin antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
pan Cadherin Antibody (2M6G8)

ICC/IF-pan Cadherin Antibody (2M6G8) [NBP3-05342] -

ICC/IF-pan Cadherin Antibody (2M6G8) [NBP3-05342] - Analysis of mouse kidney cells using E-Cadherin Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for pan Cadherin Antibody (0J8Q6) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cadherin

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Long Name

Cadherin Pan Specific

Alternate Names

Arc-1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDHE, cell-CAM 120/80, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVOE-Cadherin, Uvomorulin

Gene Symbol

CDH1

Additional Cadherin Products

Product Documents for pan Cadherin Antibody (0J8Q6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for pan Cadherin Antibody (0J8Q6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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