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Pancreatic Polypeptide/PP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1793

Novus Biologicals, part of Bio-Techne
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NB100-1793
NB100-1793-25ug

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-TRPRYGKRHKEDT corresponding to internal region according to NP_002713.1.

Reactivity Notes

Monkey and Mouse reactivity reported from verified customer reviews.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Pancreatic Polypeptide/PP Antibody

Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunocytochemistry/Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Histologic comparison of wild type and Seriola dumerili mouse pancreata. Seriola dumerili Ins2 mice (bottom) have normal islet morphology and cyto-architecture compared to littermates with endogenous mouse Ins 1 and Ins 2 (top); pancreatic polypeptide (green; B,E). Scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-40768-3) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793]

Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Pancreatic Polypeptide/PP Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

3 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23221614). Use in immunoblotting reported in scientific literature (PMID: 27572106).

Reviewed Applications

Read 3 reviews rated 5 using NB100-1793 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pancreatic Polypeptide/PP

Gene ID: 5539 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

Alternate Names

INN, Obinepitide, PNP, PP, PPY

Entrez Gene IDs

5539 (Human)

Gene Symbol

PPY

Additional Pancreatic Polypeptide/PP Products

Product Documents for Pancreatic Polypeptide/PP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pancreatic Polypeptide/PP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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