Pancreatic Polypeptide/PP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1793
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Human, Mouse
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA
Cited:
IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-TRPRYGKRHKEDT corresponding to internal region according to NP_002713.1.
Reactivity Notes
Monkey and Mouse reactivity reported from verified customer reviews.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Pancreatic Polypeptide/PP Antibody
Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793]
Immunocytochemistry/Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Histologic comparison of wild type and Seriola dumerili mouse pancreata. Seriola dumerili Ins2 mice (bottom) have normal islet morphology and cyto-architecture compared to littermates with endogenous mouse Ins 1 and Ins 2 (top); pancreatic polypeptide (green; B,E). Scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-40768-3) licensed under a CC-BY license.Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793]
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793]
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for Pancreatic Polypeptide/PP Antibody
Application
Recommended Usage
Immunohistochemistry
5 ug/mL
Immunohistochemistry-Frozen
1:500
Immunohistochemistry-Paraffin
3 ug/ml
Peptide ELISA
Detection limit 1:32000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23221614). Use in immunoblotting reported in scientific literature (PMID: 27572106).
Reviewed Applications
Read 3 reviews rated 5 using NB100-1793 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Pancreatic Polypeptide/PP
Additional Pancreatic Polypeptide/PP Products
Product Documents for Pancreatic Polypeptide/PP Antibody
Product Specific Notices for Pancreatic Polypeptide/PP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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