Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9

Format

BSA Free

Concentration

1.0 mg/ml

View all Pappalysin-1/PAPP-A Antibodies »

Product Summary for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

Immunogen

Purified human PAPP-A

Epitope

The epitope is located on the PAPP-A subunit of the heterotetramer.

Specificity

Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]

Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]

Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in paraffin embedded human placenta tissue.
Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]

Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (9) - BSA Free [NBP1-05116]

Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in frozen human placenta tissue.

Applications for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

Application
Recommended Usage

ELISA

1:8000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
ELISA: In sandwich ELISA the optimal combination is NBP1-05116 used as coating antibody and biotinylated NBP1-05043 used as detection antibody. NBP1-05116 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. WB: NBP1-05116 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of NBP2-21614 and NBP1-05043. IHC: NBP1-05116 can be used in IHC.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Pappalysin-1/PAPP-A

Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down's syndrome (trisomy 21).

Long Name

Pregnancy-associated Plasma Protein 1

Alternate Names

ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1

Gene Symbol

PAPPA

Additional Pappalysin-1/PAPP-A Products

Product Documents for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Pappalysin-1/PAPP-A Antibody (9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.