PAR/pADPr Antibody (10H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89039

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 10H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAR/pADPr Antibodies »

Product Specifications

Immunogen

Purified poly(ADP-ribose).

Specificity

Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Description

Protein A-affinity purified from supernatant.

Scientific Data Images for PAR/pADPr Antibody (10H)

Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]

Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]

Immunocytochemistry/Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039] - Detection of DNA damage.
Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]

Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]

Immunocytochemistry/Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039] - Detection of apoptotic cells by immunocytochemistry

Applications for PAR/pADPr Antibody (10H)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20 ug/ml

Immunohistochemistry-Paraffin

5-20 ug/ml
Application Notes
Immunohistochemistry (paraffin sections): dilution buffer: 5% milk (non fat dried milk) in PBS to a final concentration of 5-20 ug/ml). Western Blot: incubate 2.5 ug/ml in PBS, 0.05% Tween20, 5% milk (non fat dried milk)).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM HEPES, pH 7.4, containing 100mM sodium chloride, 1% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAR/pADPr

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Long Name

Poly [ADP-ribose] Polymer

Alternate Names

PAR

Additional PAR/pADPr Products

Product Documents for PAR/pADPr Antibody (10H)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAR/pADPr Antibody (10H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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