PAR2 Antibody (9X8E6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16555

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-16555-100ul
NBP3-16555-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9X8E6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAR2 Antibodies »

Product Summary for PAR2 Antibody (9X8E6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 298-397 of human PAR2 (P55085). ICFTPSNLLLVVHYFLIKSQGQSHVYALYIVALCLSTLNSCIDPFVYYFVSHDFRDHAKNALLCRSVRTVKQMQVSLTSKKHSRKSSSYSSSSTTVKTSY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAR2 Antibody (9X8E6)

Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555]

Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555]

Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of various cell lines, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: PAR2 Antibody (9X8E6) [NBP3-16555]

Immunohistochemistry-Paraffin: PAR2 Antibody (9X8E6) [NBP3-16555]

Immunohistochemistry-Paraffin: PAR2 Antibody (9X8E6) [NBP3-16555] - Human colon carcinoma using PAR2 Rabbit mAb (NBP3-16555) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555]

Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555]

Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of Rat large intestine, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for PAR2 Antibody (9X8E6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAR2

The Proteinase-activated receptor 2 (PAR2) is a member of the proteinase-activated receptor subfamily. It is activated through proteolytic exposure of an occult tethered ligand by trypsin and trypsin-like proteases. This is in contrast to other members of the subfamily which are activated by the protease thrombin. PAR2 has been implicated in acute inflammatory response, asthma, and pain transmission. PAR2 expression has been documented in the periphery. ESTs have been isolated from adrenal, brain, breast, heart/melanocyte/uterus, kidney, lung, and vessel libraries.

Long Name

Protease-Activated Receptor 2

Alternate Names

CFIIRL1, F2RL1, GPCR11

Gene Symbol

F2RL1

Additional PAR2 Products

Product Documents for PAR2 Antibody (9X8E6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PAR2 Antibody (9X8E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.